Archive · 13 Dec 2025
Scientists engineer glowing CRISPR protein for real-time gene editing
Scientists in Kolkata have created a special protein called GlowCas9 that glows while it edits genes.
This helps them watch the process in real-time without harming the cells.
The protein is more stable and precise, which is important for treating genetic diseases like sickle cell anemia and muscular dystrophy.
It can also be used in plants to improve crops.
The glowing effect comes from a special enzyme that lights up when the protein folds correctly, similar to fireflies.
This breakthrough could make gene therapy more effective and affordable.
Scientists from the Bose Institute in Kolkata engineered GlowCas9, a CRISPR protein that glows during gene editing.
GlowCas9 allows real-time observation of gene editing in living cells without destroying them.
The protein is more stable and precise, enhancing the success of gene therapy for genetic diseases.
GlowCas9 can be tracked in plant systems, hinting at safe, non-transgenic applications in crop improvement.
The glowing effect is due to a split nano-luciferase enzyme derived from deep-sea shrimp proteins.
- Who
- Scientists from the Bose Institute in Kolkata
- What
- Engineered a glowing CRISPR protein called GlowCas9
- When
- The study was published in the journal Angewandte Chemie International Edition
- Where
- Bose Institute, Kolkata, India
- Why
- To observe gene editing in real-time and improve the treatment of genetic diseases and cancer
- Institution
- Bose Institute, Kolkata
- Lead Researcher
- Dr. Basudeb Maji
- CRISPR Protein
- GlowCas9
- Journal
- Angewandte Chemie International Edition
- Applications
- Gene therapy, crop improvement